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To determine whether the Pro32Pro33 mutation influences the αvβ3 receptor–SERT interaction in midbrain synapses, we done coimmunoprecipitation experiments.
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The context-dependent character of SERT regulation is underscored via the lack of colocalization of SERT with focal adhesion proteins in KI synapses. This means that the association of SERT with integrin signaling complexes is just not needed for the regulation from the transporter by constitutive signaling downstream from the “primed” Pro32Pro33 mutant.
screening using the Holm–Sidak system with α < 0.05. Exams where by two unbiased variables had been assayed ended up analyzed using a two-way ANOVA and Bonferroni corrections for many testing between types (indicated in determine legends). Remaining information were being analyzed employing an unequal variance Welch t
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), suggesting that constitutive activation of integrin β3 decreases the association of SERT with integrin adhesion complexes.
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: raw densitometry values have been normalized to the common of WT values for each specialized replicate. Number of technical replicates, Pro33 Slot three. All info were being analyzed using unpaired t
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